Xenopus Development

  • Filename: xenopus-development.
  • ISBN: 9781118492826
  • Release Date: 2014-03-19
  • Number of pages: 424
  • Author: Malgorzata Kloc
  • Publisher: John Wiley & Sons

Frogs from the genus Xenopus have long been used as model organisms in basic and biomedical research. These frogs have helped unlock key fundamental developmental and cellular processes that have led to important scientific breakthroughs and have had practical application in embryology, cancer research and regenerative medicine. Xenopus Development is a vital resource on the biology and development of these key model organisms, and will be a great tool to researchers using these frogs in various disciplines of biological science. Xenopus Development is divided into four sections, the first three highlight key processes in Xenopus development from embryo to metamophosis. These sections focus on the cellular processes, organogenesis and embryo development. The final section highlights novel techniques and approaches being used in Xenopus research. Providing thorough and detailed coverage, Xenopus Development, will be a timely and welcome volume for those working in cell and molecular biology, genetics, developmental biology and biomedical research. Provides broad overview of the developmental biology of both Xenopus laevis and Xenopus tropicalis Explores cellular to systems development in key biomedical model organisms Timely synthesis of the field of Xenopus biology Highlights key biomedical and basic biological findings unlocked by Xenopus

Atlas of Xenopus Development

  • Filename: atlas-of-xenopus-development.
  • ISBN: 9788847029057
  • Release Date: 2013-06-29
  • Number of pages: 95
  • Author: G. Bernardini
  • Publisher: Springer Science & Business Media

Developmental biology attracts scientists from many different areas of biology, and the amphibian Xenopus holds a special place among the organisms studied as a model of vertebrate development. Until now, no recently published atlas existed to aid researchers and students coming to the Xenopus embryo for the first time. The present book satisfies this need. With its synthesizing approach and its generous provision of beautiful scanning, transmission, and light microscopy images, this unique volume will be a standard reference not only for developmental biologists but for all students of embryology, histology, and comparative anatomy.

Early Development of Xenopus Laevis

  • Filename: early-development-of-xenopus-laevis.
  • ISBN: 0879699426
  • Release Date: 2010-02-28
  • Number of pages: 338
  • Author: Hazel L. Sive
  • Publisher:

Amphibian embryos are supremely valuable in studies of early vertebrate development because they are large, handle easily, and can be obtained at many interesting stages. And of all the amphibians available for study, the most valuable is Xenopus laevis, which is easy to keep and ovulates at any time of year in response to simple hormone injections. Xenopus embryos have been studied for years but this is a particularly exciting time for the field. Techniques have become available very recently that permit a previously impossible degree of manipulation of gene expression in intact embryos, as well as the ability to visualize the results of such manipulation. As a result, a sophisticated new understanding of Xenopus development has emerged, which ensures the species' continued prominent position among the organisms favored for biological investigation. This manual contains a comprehensive collection of protocols for the study of early development in Xenopus embryos. It is written by several of the field's most prominent investigators in the light of the experience they gained as instructors in an intensive laboratory course taught at Cold Spring Harbor Laboratory since 1991. As a result it contains pointers, hints, and other technical knowledge not readily available elsewhere. This volume is essential reading for all investigators interested in the developmental and cell biology of Xenopus and vertebrates generally. Many of the techniques described here are illustrated in an accompanying set of videotapes which are cross-referenced to the appropriate section of the manual.

Molecular Methods in Developmental Biology

  • Filename: molecular-methods-in-developmental-biology.
  • ISBN: 9781592596782
  • Release Date: 1999-01-01
  • Number of pages: 217
  • Author: Matthew Guille
  • Publisher: Springer Science & Business Media

In Molecular Methods in Developmental Biology: Xenopus and Zebrafish, Matthew Guille assembles a hands-on collection of basic and essential molecular and embryological techniques for studying Xenopus and zebrafish. Easily reproducible and designed to succeed, these detailed methods include cellular techniques, techniques for the quantitative and spatial analysis of mRNA and proteins, and techniques for the expression of gene products in embryos. More specialized methods enable users to analyze promoters and transcription factors during early development, and include gel shift assays, as well as in vitro and in vivo footprinting. Wherever possible, these experimental approaches are applied to both Xenopus and zebrafish. Molecular Methods in Developmental Biology: Xenopus and Zebrafish affords newcomers rapid access to a wide variety of key techniques in developmental research, and offers experienced investigators both new techniques from experts who have fine-tuned them for best results, and a plethora of time-saving tips. State-of-the-art and readily reproducible, these powerful methods constitute today's gold-standard laboratory manual for understanding the interactions responsible for development.

The Early Development of Xenopus Laevis

  • Filename: the-early-development-of-xenopus-laevis.
  • ISBN: 3540537406
  • Release Date: 1991-01-01
  • Number of pages: 142
  • Author: Peter Hausen
  • Publisher: Verlag Der Zeitschrift F'Ur Naturforschung

Xenopus laevis is the key model for developmental, differential and embryological studies in vertebrates. Using molecular biological techniques it has become possible within the last few years to analyze developmental processes up to the level of genes. On obtaining results it is necessary to correlate them with morphological and histological stages. This excellent work documents and describes such developmental stages in finite detail. After an informative introduction to The Early Development of Xenopus laevis follows the highlight of the book, namely the 42 plates, each consisting of at least four high resolution micrographs. The reader is thus provided with a histological presentation of immaculate quality. Each plate is complemented by a schematic diagram describing the details shown on the plate, an additional scheme indicating the section and an explanatory short text, making this a potentially highly sought after work.

Post Transcriptional Regulation of Early Xenopus Laevis Development

  • Filename: post-transcriptional-regulation-of-early-xenopus-laevis-development.
  • ISBN: 9780549783428
  • Release Date: 2008
  • Number of pages: 108
  • Author: Jered V. McGivern
  • Publisher: ProQuest

These post transcriptional mechanisms regulate very robust and complicated developmental processes. This thesis not only lays the foundation for examining these processes in further detail, but also provides valuable information for the current understanding of these post transcriptional events during early vertebrate development.

Molecular Methods in Developmental Biology

  • Filename: molecular-methods-in-developmental-biology.
  • ISBN: 0896037908
  • Release Date: 1999-08-03
  • Number of pages: 217
  • Author: Matt Guille
  • Publisher: Humana Press

The process whereby a single cell, the fertilized egg, develops into an adult has fascinated for centuries. Great progress in understanding that process, h- ever, has been made in the last two decades, when the techniques of molecular biology have become available to developmental biologists. By applying these techniques, the exact nature of many of the interactions responsible for forming the body pattern are now being revealed in detail. Such studies are a large, and it seems ever-expanding, part of most life-science groups. It is at newcomers to this field that this book is primarily aimed. A number of different plants and animals serve as common model org- isms for developmental studies. In Molecular Methods in Developmental Bi- ogy: Xenopus and Zebrafish, a range of the molecular methods applicable to two of these organisms are described, these are the South African clawed frog, Xenopus laevis, and the zebrafish, Brachydanio rerio. The embryos of both of these species develop rapidly and externally, making them particularly suited to investigations of early vertebrate development. However, both Xenopus and zebrafish have their own advantages and disadvantages. Xenopus have large, robust embryos that can be manipulated surgically with ease, but their pseudotetraploidy and long generation time make them unsuitable candidates for genetics. This disadvantage may soon be overcome by using the diploid Xenopus tropicalis, and early experiments are already underway. The transp- ent embryos of zebrafish render them well-suited for in situ hybridization and immunohistochemistry, and good for observing mutations in genetic screens.

Assessing the Roles of XNkx2 Genes in Xenopus Cardiac Development

  • Filename: assessing-the-roles-of-xnkx2-genes-in-xenopus-cardiac-development.
  • ISBN: 9780549751229
  • Release Date: 2008
  • Number of pages: 169
  • Author:
  • Publisher: ProQuest

My aim was to gain further insight into the mechanisms of how the different Nkx2 genes influence cardiac development. I did this by examining the morphological and molecular events occurring in Xenopus laevis .

Wnt6 Function in Eye Development in Xenopus Laevis

  • Filename: wnt6-function-in-eye-development-in-xenopus-laevis.
  • ISBN: OCLC:847538163
  • Release Date: 2012
  • Number of pages:
  • Author: Sabrine Schulze
  • Publisher:

The eyes are the most important sensory organs for most vertebrates. Their structure and development is conserved between several vertebrate species. The development is regulated by several signalling pathways, including the Wnt/[beta]-catenin signalling pathway. It is required for several aspects of retinal development and it is known to regulate the proliferation of neuro-epithelial stem cells. In Xenopus laevis the intracellular Wnt/[beta]-catenin signalling pathway is activated in the retina by the Wnt receptor Fz5. Fz5 function in the eye was shown to regulate tissue specific gene expression and neuron versus Müller glial cell differentiation. However, no candidate Wnt ligand that could act through the Fz5 receptor in this tissue had been described. Wnt6 was recently found to be expressed in the developing retina, indicating that Wnt6 and Fz5 share temporal and spatial expression. Here, I tested the hypothesis that Wnt6 might function as ligand for Fz5 in the retina. In this thesis I show that a knock down of Wnt6 led to the same eye phenotype seen in Fz5 morphants, including reduced eye size, changed marker gene expression and altered neuron/Müller glia ratio. Rescue experiments show that the observed phenotype is specific and is mediated by altered Wnt/[beta]-catenin signalling pathway function. These findings support a linear model, in which Wnt6 signal interacts with the Fz5 receptor to activate the Wnt/[beta]-catenin pathway to regulate neural and Müller glia cell differentiation in retinal tissue. These results make Wnt6 a candidate for Fz5 ligand.

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