The Zebrafish

  • Filename: the-zebrafish.
  • ISBN: 9781107621343
  • Release Date: 2013-01-21
  • Number of pages: 147
  • Author: Joseph A. Holden
  • Publisher: Cambridge University Press



A full-color photomicrographic atlas allowing rapid and accurate identification of zebrafish anatomic structures at both the gross and microscopic level.

The Zebrafish Cellular and Developmental Biology

  • Filename: the-zebrafish-cellular-and-developmental-biology.
  • ISBN: 0123848938
  • Release Date: 2010-12-24
  • Number of pages: 366
  • Author:
  • Publisher: Academic Press



This volume of Methods in Cell Biology, the first of 3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases. * Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls * Summarizes the Zebrafish Genome Project

Zebrafish

  • Filename: zebrafish.
  • ISBN: 019963808X
  • Release Date: 2002-09-19
  • Number of pages: 303
  • Author: Christiane Nusslein-Volhard
  • Publisher: Oxford University Press



The zebrafish has become one of the most important model organisms to study biological processes within a living body. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease. This book not only provides a complete set of instructions that will allow researchers to establish the zebrafish in their laboratory. It also gives a broad overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques.

Cancer and Zebrafish

  • Filename: cancer-and-zebrafish.
  • ISBN: 9783319306544
  • Release Date: 2016-05-10
  • Number of pages: 552
  • Author: David M. Langenau
  • Publisher: Springer



This volume focuses on defining the unique attributes of using the zebrafish cancer model for discovering important pathways and potential drug targets for the treatment of human cancers. Using the zebrafish model, the volume explores oncogene and tumor suppressor discovery, chemical genetic approaches, genomics, epigenetics, cancer imaging, and cell transplantation. Contributed chapters come from the most prominent laboratories working in this field, which provides a unique perspective on zebrafish models from a wide spectrum of the research community. In addition, the book offers a detailed analysis of the most current research in the area for specific zebrafish cancer models, including T cell leukemia, rhabdomyosarcoma, liver and pancreatic cancer, melanoma, neuroblastoma, germ cell tumors, and malignant peripheral sheath tumors. A chapter is also dedicated to the development and utilization of other piscine models of cancer. The compilation of chapters in the volume culminates into a comprehensive and definitive text on zebrafish and cancer, providing a much needed resource on the powerful attributes of the zebrafish model system.

The Zebrafish

  • Filename: the-zebrafish.
  • ISBN: 9780123813206
  • Release Date: 2011
  • Number of pages: 568
  • Author: H. William Detrich
  • Publisher: Academic Press



This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish. * Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls * This volume concentrates on Disease Models and Chemical Screens

The Zebrafish Biology

  • Filename: the-zebrafish-biology.
  • ISBN: 9780080859576
  • Release Date: 1998-12-03
  • Number of pages: 391
  • Author:
  • Publisher: Academic Press



This is the first in a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features * Details state-of-the-art zebrafish protocols in a single-source reference * Presents methods and reagents in user-friendly format * Delineates critical steps and pitfalls of he procedures * Illustrates techniques with full-color plates * Summarizes many new and interesting developmental mutants * Includes appendices with strain information and a compendium of zebrafish World Wide Web sites * Relevant to clinicians interested in vertebrate models of human congenital diseases

The Zebrafish Genetics and Genomics

  • Filename: the-zebrafish-genetics-and-genomics.
  • ISBN: 9780080859583
  • Release Date: 1998-12-03
  • Number of pages: 396
  • Author:
  • Publisher: Academic Press



This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features * Details state-of-the-art zebrafish protocols in a single-source reference * Presents methods and reagents in user-friendly format * Delineates critical steps and pitfalls of he procedures * Illustrates techniques with full-color plates * Summarizes many new and interesting developmental mutants * Includes appendices with strain information and a compendium of zebrafish World Wide Web sites * Relevant to clinicians interested in vertebrate models of human congenital diseases

The Zebrafish Genetics Genomics and Informatics

  • Filename: the-zebrafish-genetics-genomics-and-informatics.
  • ISBN: 9780080522517
  • Release Date: 2004-12-30
  • Number of pages: 630
  • Author:
  • Publisher: Academic Press



This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 1999. This second volume covers advances in forward and reverse genetic techniques, provides an update on the zebrafish genome and gene/mutant mapping technologies, examines the new systems for efficient transgenesis in the zebrafish, provides an in-depth view of informatics and the emerging field of comparative genomics, and considers the extensive infrastructure now available to the zebrafish community. * Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls * Illustrates many techiques in full-color * Summarizes the Zebrafish Genome Project

Chemical Anatomy of the Zebrafish Retina

  • Filename: chemical-anatomy-of-the-zebrafish-retina.
  • ISBN: 1402070861
  • Release Date: 2002-08-31
  • Number of pages: 106
  • Author: Stephen Yazulla
  • Publisher: Springer Science & Business Media



Chemical Anatomy of the Zebrafish Retina is an excellent reference, providing excellent images and insight into one of the most increasingly important model systems in neurobiology today. Papers include a visually stunning molecular phenotype atlas and an exhaustive treatment of the neurochemical anatomy of the zebrafish retina as determined by immunocytochemistry.

The Zebrafish

  • Filename: the-zebrafish.
  • ISBN: 9781107621343
  • Release Date: 2013-01-21
  • Number of pages: 147
  • Author: Joseph A. Holden
  • Publisher: Cambridge University Press



A full-color photomicrographic atlas allowing rapid and accurate identification of zebrafish anatomic structures at both the gross and microscopic level.

The Zebrafish Disease Models and Chemical Screens

  • Filename: the-zebrafish-disease-models-and-chemical-screens.
  • ISBN: 9780123813213
  • Release Date: 2011-09-09
  • Number of pages: 616
  • Author:
  • Publisher: Academic Press



This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish. Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls This volume concentrates on Disease Models and Chemical Screens

Cellular and Molecular Mechanisms of Neurite Targeting in the Zebrafish Visual System

  • Filename: cellular-and-molecular-mechanisms-of-neurite-targeting-in-the-zebrafish-visual-system.
  • ISBN: 9780549488125
  • Release Date: 2008
  • Number of pages: 130
  • Author:
  • Publisher: ProQuest



Histologically discrete, parallel layers occur frequently in the nervous system. In many cases, each lamina is a target for innervation by a subset of neurons. We are interested in how neurites select their target laminae. Young zebrafish larvae develop two laminated neuropils in the visual system: the inner plexiform layer (IPL) of the retina and the synaptic region of the optic tectum. Using cell type-specific markers, we have characterized the IPL and tectal neuropil in detail, identifying the complement of neurites that compose each lamina. Using these maps, we investigated of the role of activity in IPL sublamination, and completed a forward genetic screen to identify molecules regulating lamination in the retina and tectum. In mammals, retinal activity is important for the sublamination of ganglion cell (GC) dendrites. Using pharmacological tools and the brudas/NSF mutant, we show that the zebrafish IPL develops in an activity-independent manner, at least until 7 dpf. Hard-wiring mechanisms may be conserved across these species, but other, activity-dependent mechanisms are not. Our screen uncovered five informative mutants. We exploited the moonraker (mra) and notorious (noto) mutants to explore the importance of cell-cell interactions in IPL development. Transplantation studies with mra demonstrate that a subset of amacrine cells (ACs) rely on cell non-autonomous cues to sublaminate. The mra locus encodes DEAD-box protein 19, an RNA helicase not previously implicated in neurite targeting. In noto, GC, AC, and bipolar cell neurites appear to branch outside their target sublaminae. These IPL defects are autonomous to GCs; GC dendrites are able to instruct the sublamination of partner neurites. In the tectal neuropil, noto GC axons and some tectal dendrites are highly disorganized. While wildtype axons project to a single tectal lamina, noto GCs can form arbors in two or three. We explored the developmental relationship between GC axons tectal dendrites by characterizing the tectum of lakritz (lak) mutants, which lack GCs. Our observations uncovered only slight defects in lak mutant tectal dendritogenesis. The grossly visible aspects of lamination in the tectum are largely GC-independent.

Characterization of the Development of the Zebrafish Olfactory System

  • Filename: characterization-of-the-development-of-the-zebrafish-olfactory-system.
  • ISBN: 9780549528548
  • Release Date: 2007
  • Number of pages: 181
  • Author:
  • Publisher: ProQuest



Through the ectopic expression of an OR coding region under the pan-olfactory neuronal OMP promoter, I show that zebrafish ORs can suppress the expression of endogenous receptor genes. This result suggests that, as observed in mammalian systems, OR-dependent suppression also occurs in fish. The suppression demonstrated by these experiments occurs even when the suppressing OR is expressed from a non-OR promoter. The transgenic expression system developed here provides the basis for dissecting the signaling mechanisms downstream of the OR that mediate suppression.

Morphological Identification of Amacrine Cells in the Zebrafish Retina

  • Filename: morphological-identification-of-amacrine-cells-in-the-zebrafish-retina.
  • ISBN: 9780549752653
  • Release Date: 2008
  • Number of pages: 70
  • Author:
  • Publisher: ProQuest



The zebrafish is a powerful vertebrate model in the study of visual neuroscience. Amacrine cells in the zebrafish retina were labeled using the DiOlistic technique, in which a gene gun delivers microcarriers coated with the lipophilic dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate) onto retinal slices. Incorporation of the dye allows for visualization of somata and dendritic processes. The cells were morphologically classified according to dendritic field widths, dendritic stratification patterns, and/or soma shape. Eighteen amacrine cell types were identified. Four Aoff types were monostratified in s1, one Aoff type was monostratified in s3, and two Aoff types ramified simultaneously in s1/s2 and s1/s3. Dendritic fields were 60-130 mum in both these groups. Six A on types were identified with dendritic processes innervating sublamina b of the IPL. Both rounded and pyriform-shaped somata were seen. Dendritic fields ranged in size from ∼0-110 mum. Of the five bistratified Abi cell types identified, one innervated s1/s4, three innervated s1/s5, and one innervated s2/s5. Dendritic fields were typically 20-140 mum. These results are important in determining the function of each morphological type and how they are affected in visual diseases.

Development of the Zebrafish as a Model for Bardet Biedl Syndrome

  • Filename: development-of-the-zebrafish-as-a-model-for-bardet-biedl-syndrome.
  • ISBN: 9780549402879
  • Release Date: 2007
  • Number of pages: 170
  • Author:
  • Publisher: ProQuest



Bardet-Biedl syndrome (BBS) is an autosomal recessive disorder characterized by obesity, retinitis pigmentosa, polydactyly, mental retardation and hypogonadism. To date, twelve BBS genes have been identified, however, the precise functions of BBS genes are yet to be unraveled. To unravel the pathophysiology of BBS, we developed the zebrafish BBS model.

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