The Zebrafish Genetics and Genomics

  • Filename: the-zebrafish-genetics-and-genomics.
  • ISBN: 9780080859583
  • Release Date: 1998-12-03
  • Number of pages: 396
  • Author:
  • Publisher: Academic Press



This is the second volume of a two-volume, comprehensive treatment of the methodologies used in researching the zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features * Details state-of-the-art zebrafish protocols in a single-source reference * Presents methods and reagents in user-friendly format * Delineates critical steps and pitfalls of he procedures * Illustrates techniques with full-color plates * Summarizes many new and interesting developmental mutants * Includes appendices with strain information and a compendium of zebrafish World Wide Web sites * Relevant to clinicians interested in vertebrate models of human congenital diseases

The Zebrafish

  • Filename: the-zebrafish.
  • ISBN: 9781139620635
  • Release Date: 2013-01-21
  • Number of pages:
  • Author: Joseph A. Holden
  • Publisher: Cambridge University Press



The zebrafish (Danio rerio) is a valuable and common model for researchers working in the fields of genetics, oncology and developmental sciences. This full-color atlas will aid experimental design and interpretation in these areas by providing a fundamental understanding of zebrafish anatomy. Over 150 photomicrographs are included and can be used for direct comparison with histological slides, allowing quick and accurate identification of the anatomic structures of interest. Hematoxylin and eosin stained longitudinal and transverse sections demonstrate gross anatomic relationships and illustrate the microscopic anatomy of major organs. Unlike much of the current literature, this book is focused exclusively on the zebrafish, eliminating the need for researchers to exclude structures that are only found in other fish.

Fish Development and Genetics

  • Filename: fish-development-and-genetics.
  • ISBN: 9812565760
  • Release Date: 2004
  • Number of pages: 675
  • Author: Zhiyuan Gong
  • Publisher: World Scientific



The zebrafish is the most important fish model in developmental andgenetic analyses. This book contains 19 review articles covering abroad spectrum of topics, from development to genetic tools. Thecontents range from early development, the role of maternal factorsand gastrulation, to tissue differentiation and organogenesis, such asdevelopment of the organizer, notochord, floor plate, nervous system, somites, muscle, skeleton and endoderm

The Zebrafish

  • Filename: the-zebrafish.
  • ISBN: 9780125441612
  • Release Date: 1999
  • Number of pages: 391
  • Author: H. William Detrich
  • Publisher: Academic Press



This is the first in a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications.

The Zebrafish Disease Models and Chemical Screens

  • Filename: the-zebrafish-disease-models-and-chemical-screens.
  • ISBN: 9780123813213
  • Release Date: 2011-09-09
  • Number of pages: 616
  • Author:
  • Publisher: Academic Press



This volume of Methods in Cell Biology is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish. Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls This volume concentrates on Disease Models and Chemical Screens

The Zebrafish

  • Filename: the-zebrafish.
  • ISBN: 9780123748140
  • Release Date: 2011
  • Number of pages: 504
  • Author: H. William Detrich
  • Publisher: Academic Press



This volume on genetics, genomics, and informatics, will cover new technologies in forward and reverse genetics, transgenesis, the zebrafish genome and mapping technologies, informatics and comparative genomics, and Infrastructure. This volume of Methods in Cell Biology will prove valuable both to seasoned zebrafish investigators as well as to those who are newly adopting the zebrafish model as part of their research armamentarium.

Zebrafish

  • Filename: zebrafish.
  • ISBN: 019963808X
  • Release Date: 2002-09-19
  • Number of pages: 303
  • Author: Christiane Nusslein-Volhard
  • Publisher: Oxford University Press



The zebrafish has become one of the most important model organisms to study biological processes within a living body. As a vertebrate that has many of the strengths of invertebrate model systems, it offers numerous advantages to researchers interested in many aspects of embryonic development, physiology and disease. This book not only provides a complete set of instructions that will allow researchers to establish the zebrafish in their laboratory. It also gives a broad overview of commonly used methods and a comprehensive collection of protocols describing the most powerful techniques.

Development of the Zebrafish as a Model for Bardet Biedl Syndrome

  • Filename: development-of-the-zebrafish-as-a-model-for-bardet-biedl-syndrome.
  • ISBN: 9780549402879
  • Release Date: 2007
  • Number of pages: 170
  • Author:
  • Publisher: ProQuest



Bardet-Biedl syndrome (BBS) is an autosomal recessive disorder characterized by obesity, retinitis pigmentosa, polydactyly, mental retardation and hypogonadism. To date, twelve BBS genes have been identified, however, the precise functions of BBS genes are yet to be unraveled. To unravel the pathophysiology of BBS, we developed the zebrafish BBS model.

Zebrafish

  • Filename: zebrafish.
  • ISBN: 9781416997078
  • Release Date: 2012-06-26
  • Number of pages: 128
  • Author: Sharon Emerson
  • Publisher: Simon and Schuster



When their rock band becomes popular, five middle schoolers use their new fame to generate awareness (and donations) for an important cause.

Cancer and Zebrafish

  • Filename: cancer-and-zebrafish.
  • ISBN: 9783319306544
  • Release Date: 2016-05-10
  • Number of pages: 552
  • Author: David M. Langenau
  • Publisher: Springer



This volume focuses on defining the unique attributes of using the zebrafish cancer model for discovering important pathways and potential drug targets for the treatment of human cancers. Using the zebrafish model, the volume explores oncogene and tumor suppressor discovery, chemical genetic approaches, genomics, epigenetics, cancer imaging, and cell transplantation. Contributed chapters come from the most prominent laboratories working in this field, which provides a unique perspective on zebrafish models from a wide spectrum of the research community. In addition, the book offers a detailed analysis of the most current research in the area for specific zebrafish cancer models, including T cell leukemia, rhabdomyosarcoma, liver and pancreatic cancer, melanoma, neuroblastoma, germ cell tumors, and malignant peripheral sheath tumors. A chapter is also dedicated to the development and utilization of other piscine models of cancer. The compilation of chapters in the volume culminates into a comprehensive and definitive text on zebrafish and cancer, providing a much needed resource on the powerful attributes of the zebrafish model system.

Cellular and Molecular Mechanisms of Neurite Targeting in the Zebrafish Visual System

  • Filename: cellular-and-molecular-mechanisms-of-neurite-targeting-in-the-zebrafish-visual-system.
  • ISBN: 9780549488125
  • Release Date: 2008
  • Number of pages: 130
  • Author:
  • Publisher: ProQuest



Histologically discrete, parallel layers occur frequently in the nervous system. In many cases, each lamina is a target for innervation by a subset of neurons. We are interested in how neurites select their target laminae. Young zebrafish larvae develop two laminated neuropils in the visual system: the inner plexiform layer (IPL) of the retina and the synaptic region of the optic tectum. Using cell type-specific markers, we have characterized the IPL and tectal neuropil in detail, identifying the complement of neurites that compose each lamina. Using these maps, we investigated of the role of activity in IPL sublamination, and completed a forward genetic screen to identify molecules regulating lamination in the retina and tectum. In mammals, retinal activity is important for the sublamination of ganglion cell (GC) dendrites. Using pharmacological tools and the brudas/NSF mutant, we show that the zebrafish IPL develops in an activity-independent manner, at least until 7 dpf. Hard-wiring mechanisms may be conserved across these species, but other, activity-dependent mechanisms are not. Our screen uncovered five informative mutants. We exploited the moonraker (mra) and notorious (noto) mutants to explore the importance of cell-cell interactions in IPL development. Transplantation studies with mra demonstrate that a subset of amacrine cells (ACs) rely on cell non-autonomous cues to sublaminate. The mra locus encodes DEAD-box protein 19, an RNA helicase not previously implicated in neurite targeting. In noto, GC, AC, and bipolar cell neurites appear to branch outside their target sublaminae. These IPL defects are autonomous to GCs; GC dendrites are able to instruct the sublamination of partner neurites. In the tectal neuropil, noto GC axons and some tectal dendrites are highly disorganized. While wildtype axons project to a single tectal lamina, noto GCs can form arbors in two or three. We explored the developmental relationship between GC axons tectal dendrites by characterizing the tectum of lakritz (lak) mutants, which lack GCs. Our observations uncovered only slight defects in lak mutant tectal dendritogenesis. The grossly visible aspects of lamination in the tectum are largely GC-independent.

Development of the Swimbladder as a Buoyancy Organ in the Zebrafish

  • Filename: development-of-the-swimbladder-as-a-buoyancy-organ-in-the-zebrafish.
  • ISBN: 0494391693
  • Release Date: 2008
  • Number of pages: 193
  • Author: Benjamin W. Lindsey
  • Publisher: ProQuest



Teleost fishes have body tissues that are denser than water, causing them to sink. To compensate for negative buoyancy, many teleosts possess a gas-filled swimbladder that provides lift, allowing these fish to attain neutral buoyancy at a specific depth. In this thesis I investigate three related problems in swimbladder-bearing fishes, using the zebrafish (Danio rerio) model: (1) the contribution of the swimbladder to animal buoyancy at different stages of larval development; (2) the behavioural response of adult zebrafish to simulated changes in depth; and (3) the effects of altered gravity on initial swimbladder inflation and development. Prior to initial swimbladder inflation zebrafish larvae were negatively buoyant and could not remain suspended in the water or execute vertical movements greater than a few centimeters. Thus, ascent towards the surface of the water for swimbladder inflation could only be achieved by larval attachment to the surrounding substrate. Following inflation, the volume of gas in the swimbladder allowed zebrafish to maintain near neutral buoyancy at all depths correlated with different stages of larval development. Adult zebrafish exposed to a simulated increase in depth of 50 cm or 100 cm H2O did not significantly increase their depth in the observation chamber over an 8 hour recording period. Zebrafish eggs and larvae exposed to short periods of simulated microgravity showed that once swimbladder inflation occurs, increases in volume and anatomical development of this organ follows a similar time course to the swimbladder of zebrafish raised under normal gravity. This work provides insight into the buoyancy contribution of the swimbladder during larval development and the importance of swimbladder inflation for the behaviour of fishes and for the use of zebrafish as a vertebrate model for gravitational biology.

The Zebrafish Cellular and Developmental Biology

  • Filename: the-zebrafish-cellular-and-developmental-biology.
  • ISBN: 0123870372
  • Release Date: 2011-06-02
  • Number of pages: 312
  • Author:
  • Publisher: Academic Press



This volume of Methods in Cell Biology, the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999. * Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls * Illustrates many techiques in full-color * Summarizes the Zebrafish Genome Project

Characterization of the Development of the Zebrafish Olfactory System

  • Filename: characterization-of-the-development-of-the-zebrafish-olfactory-system.
  • ISBN: 9780549528548
  • Release Date: 2007
  • Number of pages: 181
  • Author:
  • Publisher: ProQuest



Through the ectopic expression of an OR coding region under the pan-olfactory neuronal OMP promoter, I show that zebrafish ORs can suppress the expression of endogenous receptor genes. This result suggests that, as observed in mammalian systems, OR-dependent suppression also occurs in fish. The suppression demonstrated by these experiments occurs even when the suppressing OR is expressed from a non-OR promoter. The transgenic expression system developed here provides the basis for dissecting the signaling mechanisms downstream of the OR that mediate suppression.

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