Synthetic Biology

  • Filename: synthetic-biology.
  • ISBN: 9789814579568
  • Release Date: 2014-04-24
  • Number of pages: 204
  • Author: Josefine Liljeruhm
  • Publisher: World Scientific



Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: “Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed ‘A Lab Manual’ such as the one that you now hold in your hands.” Read about the interview with the authors! Contents:Introduction:What is Synthetic Biology, Exactly?The iGEM OutbreakA Synthetic Biology Lab ManualGenes, Chromoproteins and Antisense RNAs:E. coli DNA: Chromosomes, Plasmids and Copy NumberCoupling of Transcription and Translation in BacteriaPromoter and Terminator for TranscriptionRibosome Binding Site (RBS)Codon BiasChromoproteinsSmall Regulatory RNAs (sRNAs)Lab Rooms and Equipment:The Physical Lab SpacesEquipmentSafety is Priority #1:FiresChemicalsBiological Safety and DisposalDangerous EquipmentLab Course Projects:Time and ResourcesProject Overview and Learning ObjectivesThe Lab NotebookLab Section 1. Preparation of Chemical Solutions and Agar PlatesLab Section 2. Coloring Bacteria by Adding a Promoter to a Chromoprotein GeneLab Section 3: Rational Engineering of Chromoprotein Expression LevelLab Section 4. Other ExperimentsThe “Dreaded” ExamProtocols:IntroductionProtocol 1. Preparation of Solutions and Agar PlatesProtocol 2. Overnight Cultures with Antibiotics, and Glycerol StocksProtocol 3. BioBrick™ 3A Assembly and Gel AnalysisProtocol 4. Agarose Gel ElectrophoresisProtocol 5. Preparation of Competent E. coli Cells Using CaCl2Protocol 6. Transformation of CaCl2-Competent E. coli CellsProtocol 7. Bacterial Re-Streaking TechniquesProtocol 8. Lysis of E. coli Cells with LysozymeProtocol 9. Polymerase Chain Reaction (PCR)Protocol 10. Inverse PCR MutagenesisProtocol 11. Colony PCRProtocol 12. Gibson AssemblyAdvanced Methods:Flow Cytometry and Cell SortingRecombination in Plasmids and the ChromosomeElectrocompetent CellsThe International Genetically Engineered Machine (iGEM) Competition:How to Start an iGEM TeamUppsala iGEM 2011 — Show Color with ColorUppsala iGEM 2012 — Resistance is FutileUppsala iGEM 2013 — Lactonutritious — It's DeliciousAppendices Readership: Students and researchers in biotechnology, cell/molecular biology and genetics. Keywords:Synthetic Biology;Genetic Engineering;DNA Cloning;Polymerase Chain Reaction;Molecular Biology;Laboratory;Biobrick;Chromoprotein;Manual;CourseReviews: It provides an introductory workflow for common techniques and would be of definite use to educators wanting to bring synthetic biology into the classroom or for motivated undergraduate students with the desire to start thir own iGEM team.” Synthetic Biology See Full Review

Synthetic Biology

  • Filename: synthetic-biology.
  • ISBN: 9814579548
  • Release Date: 2014-06
  • Number of pages: 204
  • Author: Josefine Liljeruhm
  • Publisher: World Scientific Publishing Company



Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: "Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed 'A Lab Manual' such as the one that you now hold in your hands." The ebook version of this title will be available in April 2014.

Synthetic Biology

  • Filename: synthetic-biology.
  • ISBN: 1783268786
  • Release Date: 2015-08
  • Number of pages: 196
  • Author: Geoff Baldwin
  • Publisher:



Preceded by: Synthetic biology / Geoff Baldwin ... [and 7 others]. [2012].

Synthetic Biology Handbook

  • Filename: synthetic-biology-handbook.
  • ISBN: 9781466568488
  • Release Date: 2016-04-06
  • Number of pages: 318
  • Author: Darren N. Nesbeth
  • Publisher: CRC Press



The Synthetic Biology Handbook explains the major goals of the field of synthetic biology and presents the technical details of the latest advances made in achieving those goals. Offering a comprehensive overview of the current areas of focus in synthetic biology, this handbook: Explores the standardisation of classic molecular bioscience approaches Addresses the societal context and potential impacts of synthetic biology Discusses the use of legacy systems as tools for new product development Examines the design and construction of de novo cells and genetic codes Describes computational methods for designing genes and gene networks Thus, the Synthetic Biology Handbook provides an accurate sense of the scope of synthetic biology today. The handbook also affords readers with an opportunity to scrutinize the underlying science and decide for themselves what aspects of synthetic biology are most valuable to their research and practice.

Forensic DNA Biology

  • Filename: forensic-dna-biology.
  • ISBN: 9780123945853
  • Release Date: 2012
  • Number of pages: 198
  • Author: Kelly M. Elkins
  • Publisher: Academic Press



A collection of forensic DNA typing laboratory experiments designed for academic and training courses at the collegiate level.

Regenesis

  • Filename: regenesis.
  • ISBN: 9780465033294
  • Release Date: 2012
  • Number of pages: 284
  • Author: George McDonald Church
  • Publisher: Basic Books



Imagine a future in which human beings have become immune to all viruses, in which bacteria can custom-produce everyday items, like a drinking cup, or generate enough electricity to end oil dependency. Building a house would entail no more work than planting a seed in the ground. These scenarios may seem far-fetched, but pioneering geneticist George Church and science writer Ed Regis show that synthetic biology is bringing us ever closer to making such visions a reality. In Regenesis, Church and Regis explorethe possibilities--and perils--of the emerging field of synthetic biology. Synthetic biology, in which living organisms are selectively altered by modifying substantial portions of their genomes, allows for the creation of entirely new species of organisms. Until now, nature has been the exclusive arbiter of life, death, and evolution; with synthetic biology, we now have the potential to write our own biological future. Indeed, as Church and Regis show, it even enables us to revisit crucial points in the evolution of life and, through synthetic biological techniques, choose different paths from those nature originally took. Such exploits will involve far more than just microbial tinkering. Full-blown genomic engineering will make possible incredible feats, from resurrecting woolly mammoths and other extinct organisms to creating mirror life forms with a molecular structure the opposite of our own. These technologies--far from the out-of-control nightmare depicted in science fiction--have the power to improve human and animal health, increase our intelligence, enhance our memory, and even extend our life span. A breathtaking look at the potential of this world-changing technology, Regenesis is nothing less than a guide to the future of life.

Gene Cloning and DNA Analysis

  • Filename: gene-cloning-and-dna-analysis.
  • ISBN: 9781444334074
  • Release Date: 2010-04-19
  • Number of pages: 336
  • Author: Terry Brown
  • Publisher: John Wiley & Sons



Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology. Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. "… the book content is elegantly illustrated and well organized in clear-cut chapters and subsections… there is a Further Reading section after each chapter that contains several key references… What is extremely useful, almost every reference is furnished with the short but distinct author's remark." –Journal of Heredity, 2007 (on the previous edition)

Molecular Cloning

  • Filename: molecular-cloning.
  • ISBN: 0879695773
  • Release Date: 2001
  • Number of pages: 2344
  • Author: Joseph Sambrook
  • Publisher: CSHL Press



The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques. The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.

Biopunk

  • Filename: biopunk.
  • ISBN: 1617230073
  • Release Date: 2012-07
  • Number of pages: 250
  • Author: Marcus Wohlsen
  • Publisher:



Wohlsen delivers a chronicle of DIY biotech scientists and their idealistic quest to democratize DNA like the Internet did information.

Illustrated Guide to Home Biology Experiments

  • Filename: illustrated-guide-to-home-biology-experiments.
  • ISBN: 9781449336554
  • Release Date: 2012-04-17
  • Number of pages: 384
  • Author: Robert Bruce Thompson
  • Publisher: "O'Reilly Media, Inc."



Experience the magic of biology in your own home lab. This hands-on introduction includes more than 30 educational (and fun) experiments that help you explore this fascinating field on your own. Perfect for middle- and high-school students and DIY enthusiasts, this full-color guide teaches you the basics of biology lab work and shows you how to set up a safe lab at home. The Illustrated Guide to Home Biology Experiments is also written with the needs of homeschoolers firmly in mind, as well as adults who are eager to explore the science of nature as a life-long hobby. To get the most from the experiments, we recommend using this guide in conjunction with a standard biology text, such as the freely downloadable CK-12 Biology (ck-12.org). Master the use of the microscope, including sectioning and staining Build and observe microcosms, soda-bottle worlds of pond life Investigate the chemistry of life from simple acids, bases, and buffers to complex carbohydrates, proteins, lipids, enzymes, and DNA Extract, isolate, and observe DNA Explore photosynthesis, osmosis, nitrogen fixation, and other life processes Investigate the cell cycle (mitosis and cytokinesis) Observe populations and ecosystems, and perform air and water pollution tests Investigate genetics and inheritance Do hands-on microbiology, from simple culturing to micro-evolution of bacteria by forced selection Gain hands-on lab experience to prepare for the AP Biology exam Through their company, The Home Scientist, LLC (thehomescientist.com/biology), the authors also offer inexpensive custom kits that provide specialized equipment and supplies you’ll need to complete the experiments. Add a microscope and some common household items and you’re good to go.

Life at the Speed of Light

  • Filename: life-at-the-speed-of-light.
  • ISBN: 9781101638026
  • Release Date: 2013-10-17
  • Number of pages: 240
  • Author: J. Craig Venter
  • Publisher: Penguin



“Venter instills awe for biology as it is, and as it might become in our hands.” —Publishers Weekly On May 20, 2010, headlines around the world announced one of the most extraordinary accomplishments in modern science: the creation of the world’s first synthetic lifeform. In Life at the Speed of Light, scientist J. Craig Venter, best known for sequencing the human genome, shares the dramatic account of how he led a team of researchers in this pioneering effort in synthetic genomics—and how that work will have a profound impact on our existence in the years to come. This is a fascinating and authoritative study that provides readers an opportunity to ponder afresh the age-old question “What is life?” at the dawn of a new era of biological engineering.

Seed Technology and Its Biological Basis

  • Filename: seed-technology-and-its-biological-basis.
  • ISBN: 0849397499
  • Release Date: 2000
  • Number of pages: 419
  • Author: Michael Black
  • Publisher: CRC Press



Recent progress in plant molecular biology has revolutionized seed technology. With the enhancement of the classical properties of seeds by genetic engineering, we have seeds that produce esoteric oils, plastics, novel starches, and more. Emerging work on seed proteins, their genes - and our understanding of seed biosynthesis - has increased enormously. Seed Technology and Its Biological Basis covers the most current advances in seed development and regulation. Edited by a renowned seed biologist with a team assembled from the most respected laboratories worldwide, Seed Technology and Its Biological Basis illustrates the commercial value of seeds as a major resource. The editors provide a sweeping overview of the current state-of-the-art in seed technology and its biological basis. The book is invaluable to researchers and professionals in both the industrial and academic sectors. The chapter authors come from such institutions as: University of Reading, United Kingdom University of Bristol, United Kingdom Washington State University, Pullman, USA University of California, Berkeley, USA CSIRO, Canberra City, Australia University of Calgary, Canada The Ohio State University, USA Germains, King's Lynn, United Kingdom BC Research, Vancouver, Canada Haywards Heath, West Sussex, United Kingdom

Biolog a celular y molecular

  • Filename: biolog-a-celular-y-molecular.
  • ISBN: 9500613743
  • Release Date: 2005
  • Number of pages: 973
  • Author: Harvey Lodish
  • Publisher: Ed. Médica Panamericana



Ofrece una introducción a las técnicas y experimentos científicos del pasado y del presente, de descubrimientos importantes que llevaron a la formación de los conceptos clave en el campo. Presenta, además, información experimental esencial con nuevos problemas de análisis de datos y perspectivas para el futuro. En esta quinta edición, los temas han sido reescritos y reorganizados de forma lógica y didáctica en siete partes que incluyen: fundamentos químicos y moleculares, organización celular y bioquímica, genética y biología molecular, señalización celular, tránsito de membrana, citoesqueleto, ciclo celular y control de la proliferación celular.En lo que respecta al diseño, el lector encontrará inéditas figuras sintetizadoras, nueva combinación de esquemas con micrografías e ilustraciones esquemáticas más consistentes, que, junto con otras innovaciones, facilitan la comprensión de la temática abordada.

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