Synthetic Biology

  • Filename: synthetic-biology.
  • ISBN: 9814579548
  • Release Date: 2014-06
  • Number of pages: 204
  • Author: Josefine Liljeruhm
  • Publisher: World Scientific Publishing Company

Synthetic Biology: A Lab Manual is the first manual for laboratory work in the new and rapidly expanding field of synthetic biology. Aimed at non-specialists, it details protocols central to synthetic biology in both education and research. In addition, it provides all the information that teachers and students from high schools and tertiary institutions need for a colorful lab course in bacterial synthetic biology using chromoproteins and designer antisense RNAs. As a bonus, practical material is provided for students of the annual international Genetically Engineered Machine (iGEM) competition. The manual is based upon a highly successful course at Sweden's Uppsala University and is coauthored by one of the pioneers of synthetic biology and two bioengineering postgraduate students. An inspiring foreword is written by another pioneer in the field, Harvard's George Church: "Synthetic biology is to early recombinant DNA as a genome is to a gene. Is there anything that SynBio will not impact? There was no doubt that the field of SynBio needed 'A Lab Manual' such as the one that you now hold in your hands." The ebook version of this title will be available in April 2014.


  • Filename: biobuilder.
  • ISBN: 9781491907535
  • Release Date: 2015-06-22
  • Number of pages: 238
  • Author: Natalie Kuldell PhD.
  • Publisher: "O'Reilly Media, Inc."

Today’s synthetic biologists are in the early stages of engineering living cells to help treat diseases, sense toxic compounds in the environment, and produce valuable drugs. With this manual, you can be part of it. Based on the BioBuilder curriculum, this valuable book provides open-access, modular, hands-on lessons in synthetic biology for secondary and post-secondary classrooms and laboratories. It also serves as an introduction to the field for science and engineering enthusiasts. Developed at MIT in collaboration with award-winning high school teachers, BioBuilder teaches the foundational ideas of the emerging synthetic biology field, as well as key aspects of biological engineering that researchers are exploring in labs throughout the world. These lessons will empower teachers and students to explore and be part of solving persistent real-world challenges. Learn the fundamentals of biodesign and DNA engineering Explore important ethical issues raised by examples of synthetic biology Investigate the BioBuilder labs that probe the design-build-test cycle Test synthetic living systems designed and built by engineers Measure several variants of an enzyme-generating genetic circuit Model "bacterial photography" that changes a strain’s light sensitivity Build living systems to produce purple or green pigment Optimize baker’s yeast to produce ?-carotene

Synthetic Biology

  • Filename: synthetic-biology.
  • ISBN: 1848168624
  • Release Date: 2012
  • Number of pages: 179
  • Author: Geoff Baldwin
  • Publisher: World Scientific Publishing Company

Synthetic Biology — A Primer gives a broad overview of the emerging field of synthetic biology and the foundational concepts on which it is built. It will be of interest to final year undergraduates, postgraduates and established researchers who are interested in learning about this exciting new field. The book introduces readers to fundamental concepts in molecular biology and engineering and then explores the two major themes for synthetic biology, namely 'bottom-up' and 'top-down' engineering approaches. 'Top-down' engineering utilises a conceptual framework of engineering and systematic design to build new biological systems by integrating robustly characterised biological parts into an existing system through the use of extensive mathematical modelling. The 'bottom-up' approach involves the design and building of synthetic protocells using basic chemical and biochemical building blocks from scratch. Exemplars of cutting-edge applications designed using synthetic biology principles are presented, including the production of novel biofuels from renewable feedstocks, microbial synthesis of pharmaceuticals and fine chemicals, and the design and implementation of biosensors to detect infections and environmental waste. The book also uses the Internationally Genetically Engineered Machine (iGEM) competition to illustrate the power of synthetic biology as an innovative research and training science. Finally, the primer includes a chapter on the ethical, legal and societal issues surrounding synthetic biology, illustrating the integration of social sciences in synthetic biology research.


  • Filename: regenesis.
  • ISBN: 9780465033294
  • Release Date: 2012
  • Number of pages: 284
  • Author: George McDonald Church
  • Publisher: Basic Books

Imagine a future in which human beings have become immune to all viruses, in which bacteria can custom-produce everyday items, like a drinking cup, or generate enough electricity to end oil dependency. Building a house would entail no more work than planting a seed in the ground. These scenarios may seem far-fetched, but pioneering geneticist George Church and science writer Ed Regis show that synthetic biology is bringing us ever closer to making such visions a reality. In Regenesis, Church and Regis explorethe possibilities--and perils--of the emerging field of synthetic biology. Synthetic biology, in which living organisms are selectively altered by modifying substantial portions of their genomes, allows for the creation of entirely new species of organisms. Until now, nature has been the exclusive arbiter of life, death, and evolution; with synthetic biology, we now have the potential to write our own biological future. Indeed, as Church and Regis show, it even enables us to revisit crucial points in the evolution of life and, through synthetic biological techniques, choose different paths from those nature originally took. Such exploits will involve far more than just microbial tinkering. Full-blown genomic engineering will make possible incredible feats, from resurrecting woolly mammoths and other extinct organisms to creating mirror life forms with a molecular structure the opposite of our own. These technologies--far from the out-of-control nightmare depicted in science fiction--have the power to improve human and animal health, increase our intelligence, enhance our memory, and even extend our life span. A breathtaking look at the potential of this world-changing technology, Regenesis is nothing less than a guide to the future of life.


  • Filename: crispr-cas.
  • ISBN: 1621821307
  • Release Date: 2016-03-23
  • Number of pages: 192
  • Author: University Jennifer Doudna
  • Publisher:

The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before. Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided. Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits.

Forensic DNA Biology

  • Filename: forensic-dna-biology.
  • ISBN: 9780123945853
  • Release Date: 2012
  • Number of pages: 198
  • Author: Kelly M. Elkins
  • Publisher: Academic Press

A collection of forensic DNA typing laboratory experiments designed for academic and training courses at the collegiate level.


  • Filename: biopunk.
  • ISBN: 1617230073
  • Release Date: 2012-07
  • Number of pages: 250
  • Author: Marcus Wohlsen
  • Publisher:

Wohlsen delivers a chronicle of DIY biotech scientists and their idealistic quest to democratize DNA like the Internet did information.

Illustrated Guide to Home Biology Experiments

  • Filename: illustrated-guide-to-home-biology-experiments.
  • ISBN: 9781449336554
  • Release Date: 2012-04-17
  • Number of pages: 384
  • Author: Robert Bruce Thompson
  • Publisher: "O'Reilly Media, Inc."

Experience the magic of biology in your own home lab. This hands-on introduction includes more than 30 educational (and fun) experiments that help you explore this fascinating field on your own. Perfect for middle- and high-school students and DIY enthusiasts, this full-color guide teaches you the basics of biology lab work and shows you how to set up a safe lab at home. The Illustrated Guide to Home Biology Experiments is also written with the needs of homeschoolers firmly in mind, as well as adults who are eager to explore the science of nature as a life-long hobby. To get the most from the experiments, we recommend using this guide in conjunction with a standard biology text, such as the freely downloadable CK-12 Biology ( Master the use of the microscope, including sectioning and staining Build and observe microcosms, soda-bottle worlds of pond life Investigate the chemistry of life from simple acids, bases, and buffers to complex carbohydrates, proteins, lipids, enzymes, and DNA Extract, isolate, and observe DNA Explore photosynthesis, osmosis, nitrogen fixation, and other life processes Investigate the cell cycle (mitosis and cytokinesis) Observe populations and ecosystems, and perform air and water pollution tests Investigate genetics and inheritance Do hands-on microbiology, from simple culturing to micro-evolution of bacteria by forced selection Gain hands-on lab experience to prepare for the AP Biology exam Through their company, The Home Scientist, LLC (, the authors also offer inexpensive custom kits that provide specialized equipment and supplies you’ll need to complete the experiments. Add a microscope and some common household items and you’re good to go.

An Optimist s Tour of the Future

  • Filename: an-optimist-s-tour-of-the-future.
  • ISBN: 9781847654335
  • Release Date: 2011-01-06
  • Number of pages: 256
  • Author: Mark Stevenson
  • Publisher: Profile Books

Mark Stevenson has been to the future a few years ahead of the rest of us - and reckons it has a lot going for it. His voyage of discovery takes him to Oxford to meet Transhumanists (they intend to live forever), to Boston where he confronts a robot with mood swings, to an underwater cabinet meeting in the Indian Ocean, and Australia to question the Outback's smartest farmer. He clambers around space planes in the Mojave desert, gets to grips with the potential of nanotechnology, delves deep into the possibilities of biotech, sees an energy renaissance on a printer, a revolution in communications, has his genome profiled, and glimpses the next stage of human evolution ... and tries to make sense of what's in store. Insightful and often very funny, An Optimist's Tour of the Future is a book that tracks one curious man's journey to find out what's in store.

An Introduction to Systems Biology

  • Filename: an-introduction-to-systems-biology.
  • ISBN: 9781584886426
  • Release Date: 2006-07-07
  • Number of pages: 320
  • Author: Uri Alon
  • Publisher: CRC Press

Thorough and accessible, this book presents the design principles of biological systems, and highlights the recurring circuit elements that make up biological networks. It provides a simple mathematical framework which can be used to understand and even design biological circuits. The textavoids specialist terms, focusing instead on several well-studied biological systems that concisely demonstrate key principles. An Introduction to Systems Biology: Design Principles of Biological Circuits builds a solid foundation for the intuitive understanding of general principles. It encourages the reader to ask why a system is designed in a particular way and then proceeds to answer with simplified models.

Seed Technology and Its Biological Basis

  • Filename: seed-technology-and-its-biological-basis.
  • ISBN: 0849397499
  • Release Date: 2000
  • Number of pages: 419
  • Author: Michael Black
  • Publisher: CRC Press

Recent progress in plant molecular biology has revolutionized seed technology. With the enhancement of the classical properties of seeds by genetic engineering, we have seeds that produce esoteric oils, plastics, novel starches, and more. Emerging work on seed proteins, their genes - and our understanding of seed biosynthesis - has increased enormously. Seed Technology and Its Biological Basis covers the most current advances in seed development and regulation. Edited by a renowned seed biologist with a team assembled from the most respected laboratories worldwide, Seed Technology and Its Biological Basis illustrates the commercial value of seeds as a major resource. The editors provide a sweeping overview of the current state-of-the-art in seed technology and its biological basis. The book is invaluable to researchers and professionals in both the industrial and academic sectors. The chapter authors come from such institutions as: University of Reading, United Kingdom University of Bristol, United Kingdom Washington State University, Pullman, USA University of California, Berkeley, USA CSIRO, Canberra City, Australia University of Calgary, Canada The Ohio State University, USA Germains, King's Lynn, United Kingdom BC Research, Vancouver, Canada Haywards Heath, West Sussex, United Kingdom

Biolog a celular y molecular

  • Filename: biolog-a-celular-y-molecular.
  • ISBN: 9500613743
  • Release Date: 2005
  • Number of pages: 973
  • Author: Harvey Lodish
  • Publisher: Ed. Médica Panamericana

Ofrece una introducción a las técnicas y experimentos científicos del pasado y del presente, de descubrimientos importantes que llevaron a la formación de los conceptos clave en el campo. Presenta, además, información experimental esencial con nuevos problemas de análisis de datos y perspectivas para el futuro. En esta quinta edición, los temas han sido reescritos y reorganizados de forma lógica y didáctica en siete partes que incluyen: fundamentos químicos y moleculares, organización celular y bioquímica, genética y biología molecular, señalización celular, tránsito de membrana, citoesqueleto, ciclo celular y control de la proliferación celular.En lo que respecta al diseño, el lector encontrará inéditas figuras sintetizadoras, nueva combinación de esquemas con micrografías e ilustraciones esquemáticas más consistentes, que, junto con otras innovaciones, facilitan la comprensión de la temática abordada.

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