Preparative Chromatography

  • Filename: preparative-chromatography.
  • ISBN: 9783527649303
  • Release Date: 2012-09-27
  • Number of pages: 566
  • Author: H. Schmidt-Traub
  • Publisher: John Wiley & Sons

Completely revised and substantially extended to reflect the developments in this fast-changing field. It retains the interdisciplinary approach that elegantly combines the chemistry and engineering involved to describe the conception and improvement of chromatographic processes. It also covers recent advances in preparative chromatographic processes for the separation of "smaller" molecules using standard laboratory equipment as well as the detailed conception of industrial chemical plants. The increase in biopharmaceutical substances is reflected by new and revised chapters on different modifications of continuous chromatography as well as ion-exchange chromatography and other separation principles widely used in biochromatography. Following an introductory section on the history of chromatography, the current state of research and the design of chromatographic processes, the book goes on to define the general terminology. There then follow sections on stationary phases, selection of chromatographic systems and process concepts. A completely new chapter deals with engineering and operation of chromatographic equipment. Final chapters on modeling and determination of model parameters as well as model based design, optimization and control of preparative chromatographic processes allow for optimal selection of chromatographic processes. Essential for chemists and chemical engineers in the chemical, pharmaceutical, and food industries.

Preparative Chromatography Techniques

  • Filename: preparative-chromatography-techniques.
  • ISBN: 9783662036310
  • Release Date: 2013-03-14
  • Number of pages: 247
  • Author: K. Hostettmann
  • Publisher: Springer Science & Business Media

Over the past few years, increasing attention has been paid to the search for bioactive compounds from natural sources. The success of plant-derived products such as paclitaxel (Taxol) in tumor therapy or artemisinin in the treatment of malaria has provided the impetus for the introduction of numerous research programmes, especially in Industry. A great deal of effort is being expended in the generation of novel lead molecules of vegetable, marine and microbial origin by the use of high throughput screening protocols. When interesting hits are found, it is essen tial to have methods available for the rapid isolation of target compounds. For this reason, both industry and academia need efficient preparative chromatographic separation techniques and experience in their application. Purified natural products are required for complete spectro scopic identification and full characterization of new compo unds, for biological testing and for the supply of pharmaceuti cals, standards, and starting materials for synthetic work. Obtaining pure products from an extract can be a very long, te dious and expensive undertaking, involving many steps. Sometimes only minute amounts of the desired compounds are at hand and these entities may be labile. Thus it is an advantage to have access to as many different methods as possible in order to aid the isolation process. Although a certain amount of trial and error may be involved, nowadays there is the possibility of devising suitable rapid separation schemes by a judicious choice of the different techniques available.

Preparative Chromatography

  • Filename: preparative-chromatography.
  • ISBN: 9783527605903
  • Release Date: 2006-03-06
  • Number of pages: 485
  • Author: H. Schmidt-Traub
  • Publisher: John Wiley & Sons

This interdisciplinary approach combines the chemistry and engineering involved to describe the conception and improvement of chromatographic processes. The book covers recent developments in preparative chromatographic processes for the separation of "smaller" molecules using standard laboratory equipment as well as the detailed conception of industrial chemical plants. Following an introductory section on the history of chromatography, the current state of research and the design of chromatographic processes, the book goes on to define the general terminology. There then follow sections on solid materials and packed columns process concepts. Final chapters on modeling and determination of model parameters, the design and optimization of preparative chromatographic processes and chromatographic reactors allow for the optimum selection of chromatographic systems. Essential for chemists and engineers working in the chemicals and pharmaceutical industries as well as for food technologies, due to the interdisciplinary nature of these processes.

Scale Up and Optimization in Preparative Chromatography

  • Filename: scale-up-and-optimization-in-preparative-chromatography.
  • ISBN: 0203909283
  • Release Date: 2002-09-26
  • Number of pages: 368
  • Author: Anurag Rathore
  • Publisher: CRC Press

Presenting guidelines to predict and improve separation system performance, this book contains numerous case studies illustrating the practice of scale-up principles in process development. It offers solutions to limitations that occur in real-world purification schemes; methods to model, optimize, and characterize nonlinear separation processes; data comparisons from all stages of production; and industrial separation schemes for products such as synthetic molecules, antibody fragments, IgG, growth factors, and plasmid DNA. The book covers external constraints, separation economics, correlations for transport and kinetic phenomena, and the configuration and parameters of column design.

Preparative and Production Scale Chromatography

  • Filename: preparative-and-production-scale-chromatography.
  • ISBN: 0824787382
  • Release Date: 1992-09-16
  • Number of pages: 816
  • Author: G. Ganetsos
  • Publisher: CRC Press

Describes the latest developments in the scaling-up and application of chromatographic operations and demonstrates that production-scale chromatography is a powerful and invaluable separation process. The book covers every important process design and reveals actual, immediately applicable techniques and is designed to appeal to design, chemical/biochemical, and research and development engineers, process development managers, bioprocess technologists, analytical and clinical chemists and biochemists, pharmacists, and upper-level undergraduate, graduate, and continuing-education students in these disciplines.

Preparative Enantioselective Chromatography

  • Filename: preparative-enantioselective-chromatography.
  • ISBN: 9781405144711
  • Release Date: 2008-04-15
  • Number of pages: 344
  • Author: Geoffrey B. Cox
  • Publisher: John Wiley & Sons

The development of chiral liquid chromatography, facilitating the straightforward separation of enantiomers, was a significant advance in chromatography, leading to widespread application in analytical chemistry. Application in preparative chromatography has been less rapid, but with the development of single enantiomer pharmaceuticals its use is increasingly common in chemical synthesis at laboratory, pilot plant and even full production scale. Brings non-experts up to speed quickly and comprehensively, facilitating the rapid development of effective separations of enantiomeric mixtures on a range of process scales Presents case studies drawn from within the pharmaceutical industry to clearly illustrate the utility and value of preparative scale enantioselective chromatography in chemical research, development and production Key reference source and entry to the literature so the reader does not have to engage in expensive and time consuming literature searching

Ion Exchange Sorption and Preparative Chromatography of Biologically Active Molecules

  • Filename: ion-exchange-sorption-and-preparative-chromatography-of-biologically-active-molecules.
  • ISBN: 9781468489088
  • Release Date: 2012-12-06
  • Number of pages: 178
  • Author: G.V. Samsonov
  • Publisher: Springer Science & Business Media

This book deals with the physico-chemical principles underlying ion exchange sorption and chromatog~aphy. It does not cover in any detail the experimental and instrumental aspects of practical separations. The author has developed the subject starting from the synthesis and structure of the ion exchangers employed, through the thermodynamics of sorption selectivity and the equilibrium dynamics of ion sorption, to the kinetics and dynamics of non-equilibrium ion-exchange systems. Throughout this treatment the additional factors arising from the exchange of complex organic ions, as opposed to simple mineral ones, have been interwoven. The author has stressed the application in the separation of organic ions with biological activity, many of which are synthesized in biotechnological processes, and in view of this he uses the expression "physico-chemical biotechnology." In practice, however, his in-depth treatment is applicable to any charged organic species with multifunction ality and/or high molecular weight, and is therefore by no means restricted to biologically active materials, and certainly not to those molecules from a biotechnological source. Bearing this in mind, the text has a much wider value than the title may convey.

Preparative Scale Chromatography

  • Filename: preparative-scale-chromatography.
  • ISBN: 0824780612
  • Release Date: 1988-10-28
  • Number of pages: 344
  • Author: Eli Grushka
  • Publisher: CRC Press

Discusses a model for the propagation of a finite concentration zone in a chromatographic column for the case of a single component sample, and explores operating conditions of ion-exchange chromatography for the production of albumin from bovine serum and plasma. Focus is on theoretical, instrument

Fundamentals of Preparative and Nonlinear Chromatography

  • Filename: fundamentals-of-preparative-and-nonlinear-chromatography.
  • ISBN: 9780123705372
  • Release Date: 2006
  • Number of pages: 975
  • Author: Georges Guiochon
  • Publisher: Academic Press

The second edition of Fundamentals of Preparative and Nonlinear Chromatographyis book that discusses all the physico-chemical issues related to linear and nonlinear chromatography. It summarizes the essential issues involved in thermodynamics (single-component and competitive liquid-solid equilibrium isotherms, influence of the temperature, the pressure, and the mobile phase composition on these isotherms, heterogeneity of the surface of adsorbents), mass transfer kinetics (diffusion through bulk liquids, through particle beds, and through particles, surface diffusion, competitive diffusion), and flow through porous media (pressure drop, permeability, viscosity of mixtures, viscous fingering). It presents the different models used in chromatography (ideal, nonlinear model, linear, nonideal model, equilibrium-dispersive model, transport and transport-dispersive models, general rate model, POR model), their algebraic and numerical solutions, and the practical applications made of these solutions (elution, frontal analysis and displacement chromatography, gradient elution, system peaks). Also discussed are the various processes derived from chromatography, particularly the different modes of recycling, the true and the simulated moving bed separators, including the recent variants of this SMB process. Finally, it describes and illustrates the different methods used for the optimization of the experimental conditions with different objective functions.

Gradient Techniques in Preparative Chromatography

  • Filename: gradient-techniques-in-preparative-chromatography.
  • ISBN: 3838310764
  • Release Date: 2009-09
  • Number of pages: 148
  • Author: Dorota Antos
  • Publisher: LAP Lambert Academic Publishing

Chromatographic separations are usually realized in isocratic mode, i.e., with constant temperature, pressure or solvent composition of the mobile phase during the process. However, it is well known that the adsorption behavior of the system can be strongly affected by changes of these operating parameters in gradient mode. In the work presented here several aspects of applying the gradient technique for preparative scale of both batch and continuous chromatography are discussed. This analysis is included in the main PART II of the work. It starts from the examination of a batch system consisting of a single column. Subsequently, strategies of using gradient in a continuous separation with a parallel multi-column arrangement and with the SMB system are discussed. Before analyzing the gradient technique the application of mathematical models of process dynamics for predicting chromatographic separations is thoroughly examined in PART I of the work. Moreover, in PART I several aspects of the application of numerical methods for solving the model equations are discussed.

Liquid Chromatography

  • Filename: liquid-chromatography.
  • ISBN: 9780128071465
  • Release Date: 2013-01-08
  • Number of pages: 520
  • Author: M. Kaspereit
  • Publisher: Elsevier Inc. Chapters

This chapter describes rivalling operating modes applied industrially in preparative chromatography. After recapitulating the basic features of the “workhorse” and reference in the field, namely, isocratic batch elution, at first, more-sophisticated concepts are presented that offer additional degrees of freedom but maintain the discontinuous character of the separation process. Subsequently, process options are described that possess the potential to perform the separations continuously. Hereby, mainly the most successful multicolumn concepts are explained, which are based on realizing highly efficient countercurrent transport processes. Finally, the important aspect is addressedL how different process alternatives can be evaluated and compared quantitatively.

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